Na(+)-K(+)-Cl- cotransporter of brain capillary endothelial cells. Properties and regulation by endothelins, hyperosmolar solutions, calyculin A, and interleukin-1.

Na + - K + - CI - Cotransporter of Brain Capillary Endothelial Cells

Cultured rat brain capillary endothelial cells expressed a large BsRb+ uptake component that was dependent on external Na+ and C1and that was inhibited by loop diuretics with unusual pharmacological properties: benzmetanide (IC, = 1-5 p ~ ) = bumetanide (ICso = 1-5 p ~ ) > piretanide (ICso = 3-16 p ~ ) = furosemide (ICso = 7-11 p ~ ) . It was activated %fold by endothelin-1 (EC, = 1 MI) and end...

Functional interaction of the K-Cl cotransporter (KCC1) with the Na-K-Cl cotransporter in HEK-293 cells.

We have studied the regulation of the K-Cl cotransporter KCC1 and its functional interaction with the Na-K-Cl cotransporter. K-Cl cotransporter activity was substantially activated in HEK-293 cells overexpressing KCC1 (KCC1-HEK) by hypotonic cell swelling, 50 mM external K, and pretreatment with N-ethylmaleimide (NEM). Bumetanide inhibited 86Rb efflux in KCC1-HEK cells after cell swelling [inhi...

The thiazide-sensitive Na+-Cl- cotransporter: molecular biology, functional properties, and regulation by WNKs.

The thiazide-sensitive Na+-Cl(-) cotransporter is the major salt reabsorption pathway in the distal convoluted tubule, which is located just after the macula densa at the beginning of the aldosterone-sensitive nephron. This cotransporter was identified at the molecular level in the early 1990s by the pioneering work of Steven C. Hebert and coworkers, opening the molecular area, not only for the...

The Thiazide - sensitive Na + : Cl - Cotransporter : molecular biology , 3 functional properties , and regulation by WNKs

13 Address correspondence to: 14 Gerardo Gamba, M.D., Ph.D. 15 Vasco de Quiroga No. 15, Tlalpan 14000 Mexico City, Mexico 16 e-mail: [email protected] or [email protected] 17 18

Shear stress and 17 -estradiol modulate cerebral microvascular endothelial Na-K-Cl cotransporter and Na/H exchanger protein levels